dextran hydrodynamic radius

The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. The range of temperatures used was 25-50 (0.1) C and it was regulated by thermostatic bath Haake C. The viscosity measurements required Hagenbach-Couette correction time (tg 37.31s). Figure4 shows the histological analysis of micropores in human cadaver skin. The fast increasing of these polyglucosans for medical, industrial and research purposes motivated a survey of the types obtainable. These studies were again focused on investigating the hydrodynamic properties of the polymer molecules, their hydrodynamic radius in relation to their radius of gyration and the coherence of the simulation results with polymer theory. California Privacy Statement, 2.It can be found from the figure that at the start-up stage of the shaft, the hydrodynamic effect (HE) is weak, so the HP is not enough to support the shaft, which European Polymer Journal, 36, 2143-2146. Microporations were performed 2, 5, and 10 times and the skin damage was confirmed using the TEWL measurement. Citation-(RIS The regeneration process of the skin is regulated by growth factors such as keratinocyte growth factor and fibroblast growth factor, which trigger the proliferation and migration of cells [23, 24]. The formula is verified for different systems of polymer-solvent and the values are in accord with those obtained by extrapolation. 43-1, 115-147. ; Weisz, J. Unperturbed dimensions and the theta temperature of dextran in ethylene glycol solutions. The genus Weissella: taxonomy, ecology and biotechnological potential. Carbohydrate Polymers, 68, 477-488. Front Microbiol. 8. Ann Surg Oncol. In our present study, microporated skin permeated insufficient amount of 20K FITC-dextran, suggesting that enhancement of permeability was unsuccessful with macromolecules of more than 20KM.W.. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Pharm Res. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. Determination of the intrinsic viscosity has been the most used measurement in the last 60 years. Carbohydrate Polymers 99, 199-207. WebHydrodynamic radius = variable size of an analyte with specific MW based on dynamical properties. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. Colloid and Polymer Science, 286, 1223-1231. For 40 kDa molecules, the hydrodynamic diameter dh ~8 nm is much smaller than the collagen mesh size [ 33, 41 ]. The cumulative permeation of FITC-dextran 10 K was 4.82 0.32 M, in the human skin and 5.92 0.46 M in the pig skin. Gillespie, T.; Hulme, M. A. For data requests, please contact the authors. Webdextran hydrodynamic radius. Cytotoxicity studies on human cervical cancer (HeLa) cell line showed non-toxic and biocompatible nature of dextran. Formation of dextran hydrogels by crystallization. Gums and stabilisers for the Food Industry, 5, 447-450. Webwhittier union high school district superintendent. Beer, M.U. (2008). Bookshelf Huggins, Maurice L. (1942). A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. dextran hydrodynamic radius. An official website of the United States government. Viswanath, D.S. The solutions were prepared in bidestilated water at 1, 0.75, 0.5, 0.25 % wt. ; Prasad, D.H.L. Study of temperature influence. Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). Figure 3 shows that the parameters vary with temperature, evidencing a clear functionality of them. Journal of American Chemical Society, 76 (20), 5041-5052. Hi Khanh, Alan is taking a little time off of Research Gate at the moment, but here is some information in reply to your question. Similar results are obtained in references [35, 36, 37], using a system of dextran / ethylene glycol, and dextran/dimethylsulfoxide, respectively. In summary, we demonstrated effective enhancement of skin permeability and delivery of macromolecules lower than 20KM.W., and regeneration of human cadaver skin after microporation with an RF-based microporation device. The particular dextrans which were used initially for conversion into synthetic blood-volume expanders [6, 7], human red blood cells aggregation for increasing the degree of polymerization and hence the molecular weight [8, 9, 10], hydrogels and microspheres [11], in drug transport system and its modifications as nanoparticles [12], in the removal through absorption of contaminant effluents such as heavy metals, organic molecules and inks [13], in the formation of biodegradable films [14, 15]. Microporation was performed 2 times on each sample. (1937). Analytical Chemistry. A lot of research has been devoted to dextrans modification in order to describe special characteristics relevant to specific applications such as surfactants [16], as visible marker [17], polysaccharides-based nanoparticles, covalent crosslinking [18], ionic crosslinking, polyelectrolyte complex, and the self-assembly of hydrophobically modified polysaccharides, and hydrophilic modified polysaccharides [19]. This study demonstrated that hydrodynamic volume and radius of gyration of a dextran sample with M w < 20 kDa and its linear counterpart with equal M w are almost The above data are not useful because it can not be compared with those obtained in this work. (2008). Data File Dextran. The Hydrodynamic Radii of Macromolecules and Their Effect on Red Blood Cell Aggregation. ; Yevlampieva N.P. The Transdermal diffusion cell system was protected from light. Table1 shows TEWL values measured immediately after the microporation of human and pig skins. WebTry out PMC Labs and tell us what you think. Format), Citation-(EndNote Format). The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC Ortega, A.; Garca de la Torre, J. Dextran consists of -D glucose units with a majority of (16) glucosidic linkages between them. Rotureau, E.; Dellacherie, E.; Durand, A. The calculation of Mark-Houwink parameters is carried out by the graphic representation of the following equation: where kMH (cm3/g) and a are Mark-Houwink constants, depending on the type of polymer, solvent, and temperature of intrinsic viscosity determinations [46]. 2012 Sep 1;90(1):441-6. doi: 10.1016/j.carbpol.2012.05.063. Tirtaatmadja, V.; Dunstan, D. E.; Boger, D. V. (2001). The most widely used method for the characterization of macromolecules is the capillary viscometry, as it is a simple and economic method. Middelkoop E, Bogaerdt AJ, Lamme EN, Hoekstra MJ, Brandsma K, Ulrich MMW. Hrabchak C, Flynn L, Woodhouse KA. The important role of thermodynamic interaction parameter in the determination of theta temperature, dextran/water system. Determination of constants, kM-H and a, from the intrinsic viscosity data, requires a series of monodisperse polymer samples with known molecular weight or a series of polydisperse polymer samples with known viscosity average molecular weights, Mv. (2013). B 113, 8020-8025. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. Drug Discov Today Technol. 2019;9(3-S):9726. Hydrodynamic radii were measured using Dynamic Light Scattering (DLS) in a Malvern Zetasizer NanoZS DLS (Malvern Instruments, Malvern, UK) at high scattering angle (173) to minimise the effects of dust and supramolecular contamination (NIBS, 173). Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation, https://doi.org/10.1186/s40824-020-00201-7, http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/. Drug deliv. ; Tsuchiya, H.M.; Rist, C.E. Polyelectrolytes as secondary calibration standards for aqueous SEC. Charge: Anionic. The relative browning for dextran from W. cibaria JAG8 was similar to commercial prebiotic Nutraflora P-95 and 3-fold lower than Raftilose P-95. Microporation applications for enhancing drug delivery. Dextran is a branched polysaccharide, composed of -D-glucopyranosyl residues. Synthesis and characterization of blue dextrans. (1969). Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. The basic setup of a DLS instrument is shown in Figure 1. Huggins equation [41] gives the relation to the relative viscosity increase (i). Dextran; Weissella cibaria; exopolysaccharide; prebiotic; skimmed milk. WebDextran 40 Pharmaceutical Quality. ; Bataille, B. Kloster, C.; Bica C.; Rochas C.; Samios, D.; Geissler, E. (2000). All authors read and approved the final manuscript. Harding, S. E.; Varum, K.; Stoke B.; Smidsrod, O. Granath, K. A. The human and pig skin samples were placed in the receiver chamber of the Transdermal diffusion cell system with the SC facing up and the donor chamber was fixed in place. (2008). Smith, David R.; Raymonda, John W. (1972). Dear Sir. Alan F Rawle , Can you help me in calculating the size of dextran 4 (molecular weight= 4000) and dextran 10 (molecular weight 10,000)? Th Chen Rong Huei; Min Lang Tsai (1998). If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. The Dextrans intrinsic viscosity is determined as a function of the molecular weight in water and NaOH 0.5 M see reference [1]. Carbohydrate Research, 340, 811-826. Treatment of acne scars by fractional bipolar radiofrequency energy. 1994, 95-0355. If there are particles in the sample, the incident laser light gets scattered in all directions. Drug Deliv. The optical image and histological analysis confirmed that the micropores on the skin were recovered over time. Carbohydrate Research, 160, 205-214. Analyzing the values of the hydrodynamic properties of dextran in aqueous solution, it can be seen in Table 7, that all vary with the temperature. The molecular weight of dextran samples can be obtained by a variety of methods including light scattering, HPLC/SEC, sedimentation, osmometry and end-group analysis. Dynamics of a Polymer Solution in a Rigid Matriz. Arch Dermatol Res. Springer Nature. DOI: 10.12691/jpbpc-1-1-3, Received September 18, 2013; Revised October 23, 2013; Accepted November 22, 2013. official website and that any information you provide is encrypted Advances in Cosmetic Surgery. The radius of gyration is typically calculated from static scattering measurements, while the hydro-dynamic radius (or equivalent sphere radius) is deter-mined by dynamic light scattering or other diffusion measurements. In order to confirm the molecular weight the gel permeation chromatography (GPC) for standard dextrans is used in a range of molecular weights from 8.8 to 2000kDa and these correspond to the following equation.